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β5c  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc β5c
    Effect of compounds 5b , <t>5c</t> , and 5j – l , as well as subunit selective control compounds on the secretion of cytokines in LPS-stimulated PBMCs. All compounds were tested at 250 nM. The cells were pretreated for 1 h with inhibitors, followed by the addition of LPS (1 μg/mL). As the negative control (designated by ‘DMSO’ in figure legends), cells were treated only with DMSO, followed by the addition of LPS (1 μg/mL). The concentrations of cytokines were determined in the supernatants after additional 24 h treatment. The results are represented as means ± SD of four independent experiments ( N = 4). Statistical significance between untreated controls versus treated was calculated using one-way ANOVA post hoc Dunnett’s test. A p -value of less than 0.05 was considered significant (**** p < 0.0001; *** p < 0.001; ** p < 0.01; * p < 0.05).
    β5c, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+%CE%B25c/PSMB5+Rabbit+mAb/pmc12751025-282-29-38
    Average 93 stars, based on 55 article reviews
    β5c - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "α‑Aminoboronic Acid Moieties in Boro Dipeptides Modulate Proteasome Subunit Selectivity and Provide Access to Compounds with Potent Anticancer and Anti-Inflammatory Activity"

    Article Title: α‑Aminoboronic Acid Moieties in Boro Dipeptides Modulate Proteasome Subunit Selectivity and Provide Access to Compounds with Potent Anticancer and Anti-Inflammatory Activity

    Journal: Journal of Medicinal Chemistry

    doi: 10.1021/acs.jmedchem.5c02548

    Effect of compounds 5b , 5c , and 5j – l , as well as subunit selective control compounds on the secretion of cytokines in LPS-stimulated PBMCs. All compounds were tested at 250 nM. The cells were pretreated for 1 h with inhibitors, followed by the addition of LPS (1 μg/mL). As the negative control (designated by ‘DMSO’ in figure legends), cells were treated only with DMSO, followed by the addition of LPS (1 μg/mL). The concentrations of cytokines were determined in the supernatants after additional 24 h treatment. The results are represented as means ± SD of four independent experiments ( N = 4). Statistical significance between untreated controls versus treated was calculated using one-way ANOVA post hoc Dunnett’s test. A p -value of less than 0.05 was considered significant (**** p < 0.0001; *** p < 0.001; ** p < 0.01; * p < 0.05).
    Figure Legend Snippet: Effect of compounds 5b , 5c , and 5j – l , as well as subunit selective control compounds on the secretion of cytokines in LPS-stimulated PBMCs. All compounds were tested at 250 nM. The cells were pretreated for 1 h with inhibitors, followed by the addition of LPS (1 μg/mL). As the negative control (designated by ‘DMSO’ in figure legends), cells were treated only with DMSO, followed by the addition of LPS (1 μg/mL). The concentrations of cytokines were determined in the supernatants after additional 24 h treatment. The results are represented as means ± SD of four independent experiments ( N = 4). Statistical significance between untreated controls versus treated was calculated using one-way ANOVA post hoc Dunnett’s test. A p -value of less than 0.05 was considered significant (**** p < 0.0001; *** p < 0.001; ** p < 0.01; * p < 0.05).

    Techniques Used: Control, Negative Control

    Related Articles

    Polyacrylamide Gel Electrophoresis:

    Article Title: Design and Evaluation of Highly Selective Human Immunoproteasome Inhibitors Reveal a Compensatory Process That Preserves Immune Cell Viability.
    Article Snippet: The pan-proteasome inhibitor bortezomib demonstrated clinical efficacy in off-label trials of Systemic Lupus Erythematosus.. One potential mechanism of this clinical benefit is from the depletion of pathogenic immune cells (plasmablasts and plasmacytoid dendritic cells).. However, bortezomib is cytotoxic against non-immune cells which limits its use for autoimmune diseases.

    Membrane:

    Article Title: Design and Evaluation of Highly Selective Human Immunoproteasome Inhibitors Reveal a Compensatory Process That Preserves Immune Cell Viability.
    Article Snippet: The pan-proteasome inhibitor bortezomib demonstrated clinical efficacy in off-label trials of Systemic Lupus Erythematosus.. One potential mechanism of this clinical benefit is from the depletion of pathogenic immune cells (plasmablasts and plasmacytoid dendritic cells).. However, bortezomib is cytotoxic against non-immune cells which limits its use for autoimmune diseases.

    Blocking Assay:

    Article Title: Design and Evaluation of Highly Selective Human Immunoproteasome Inhibitors Reveal a Compensatory Process That Preserves Immune Cell Viability.
    Article Snippet: The pan-proteasome inhibitor bortezomib demonstrated clinical efficacy in off-label trials of Systemic Lupus Erythematosus.. One potential mechanism of this clinical benefit is from the depletion of pathogenic immune cells (plasmablasts and plasmacytoid dendritic cells).. However, bortezomib is cytotoxic against non-immune cells which limits its use for autoimmune diseases.



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    Effect of compounds 5b , <t>5c</t> , and 5j – l , as well as subunit selective control compounds on the secretion of cytokines in LPS-stimulated PBMCs. All compounds were tested at 250 nM. The cells were pretreated for 1 h with inhibitors, followed by the addition of LPS (1 μg/mL). As the negative control (designated by ‘DMSO’ in figure legends), cells were treated only with DMSO, followed by the addition of LPS (1 μg/mL). The concentrations of cytokines were determined in the supernatants after additional 24 h treatment. The results are represented as means ± SD of four independent experiments ( N = 4). Statistical significance between untreated controls versus treated was calculated using one-way ANOVA post hoc Dunnett’s test. A p -value of less than 0.05 was considered significant (**** p < 0.0001; *** p < 0.001; ** p < 0.01; * p < 0.05).
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    Effect of compounds 5b , <t>5c</t> , and 5j – l , as well as subunit selective control compounds on the secretion of cytokines in LPS-stimulated PBMCs. All compounds were tested at 250 nM. The cells were pretreated for 1 h with inhibitors, followed by the addition of LPS (1 μg/mL). As the negative control (designated by ‘DMSO’ in figure legends), cells were treated only with DMSO, followed by the addition of LPS (1 μg/mL). The concentrations of cytokines were determined in the supernatants after additional 24 h treatment. The results are represented as means ± SD of four independent experiments ( N = 4). Statistical significance between untreated controls versus treated was calculated using one-way ANOVA post hoc Dunnett’s test. A p -value of less than 0.05 was considered significant (**** p < 0.0001; *** p < 0.001; ** p < 0.01; * p < 0.05).
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    Effect of compounds 5b , <t>5c</t> , and 5j – l , as well as subunit selective control compounds on the secretion of cytokines in LPS-stimulated PBMCs. All compounds were tested at 250 nM. The cells were pretreated for 1 h with inhibitors, followed by the addition of LPS (1 μg/mL). As the negative control (designated by ‘DMSO’ in figure legends), cells were treated only with DMSO, followed by the addition of LPS (1 μg/mL). The concentrations of cytokines were determined in the supernatants after additional 24 h treatment. The results are represented as means ± SD of four independent experiments ( N = 4). Statistical significance between untreated controls versus treated was calculated using one-way ANOVA post hoc Dunnett’s test. A p -value of less than 0.05 was considered significant (**** p < 0.0001; *** p < 0.001; ** p < 0.01; * p < 0.05).
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    Image Search Results


    Effect of compounds 5b , 5c , and 5j – l , as well as subunit selective control compounds on the secretion of cytokines in LPS-stimulated PBMCs. All compounds were tested at 250 nM. The cells were pretreated for 1 h with inhibitors, followed by the addition of LPS (1 μg/mL). As the negative control (designated by ‘DMSO’ in figure legends), cells were treated only with DMSO, followed by the addition of LPS (1 μg/mL). The concentrations of cytokines were determined in the supernatants after additional 24 h treatment. The results are represented as means ± SD of four independent experiments ( N = 4). Statistical significance between untreated controls versus treated was calculated using one-way ANOVA post hoc Dunnett’s test. A p -value of less than 0.05 was considered significant (**** p < 0.0001; *** p < 0.001; ** p < 0.01; * p < 0.05).

    Journal: Journal of Medicinal Chemistry

    Article Title: α‑Aminoboronic Acid Moieties in Boro Dipeptides Modulate Proteasome Subunit Selectivity and Provide Access to Compounds with Potent Anticancer and Anti-Inflammatory Activity

    doi: 10.1021/acs.jmedchem.5c02548

    Figure Lengend Snippet: Effect of compounds 5b , 5c , and 5j – l , as well as subunit selective control compounds on the secretion of cytokines in LPS-stimulated PBMCs. All compounds were tested at 250 nM. The cells were pretreated for 1 h with inhibitors, followed by the addition of LPS (1 μg/mL). As the negative control (designated by ‘DMSO’ in figure legends), cells were treated only with DMSO, followed by the addition of LPS (1 μg/mL). The concentrations of cytokines were determined in the supernatants after additional 24 h treatment. The results are represented as means ± SD of four independent experiments ( N = 4). Statistical significance between untreated controls versus treated was calculated using one-way ANOVA post hoc Dunnett’s test. A p -value of less than 0.05 was considered significant (**** p < 0.0001; *** p < 0.001; ** p < 0.01; * p < 0.05).

    Article Snippet: Membranes were blocked with 5% BSA in 1 × TTBS for 1 h at room temperature and incubated overnight at 4 °C with primary antibodies against: β5i (1:1000; #13726), β5c (1:1000; #12919), and β1c (1:1000; #13267; all from Cell Signaling Technology), β1i (1:1000; #ab187645; all from Abcam), β2c (1:1000, #ab154745), and β2i (1:1000, #ab183506), and β-actin (1:5000; #A5316; SigmaPrestige).

    Techniques: Control, Negative Control